Interactive Effects of Endurance Swimming and Curcumin Supplementation on Serum Levels of Liver Alkaline Phosphatase in Male Rats Following Ethanol Abuse

Document Type : Original research papers

Authors

1 Department of Physical Education and Sport Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran.

2 Department of Physical Education ,Borujerd Branch,Islamic Azad University, Borujerd,Iran

3 Department of Sport Physiology, Faculty of Sport Sciences, Bu Ali Sina University, Hamedan, Iran.

Abstract

In this study, 48 male wistar rats approximately 12 weeks old with  240 ±10g weight are selected.(1st) 8 rats were randomly separated as control group and 40 rats in 5 equal group were gavaged with ethanol (5-7 g/kg weight of body) every 8 hours for 4 days. Six days after the final binge, (2nd) the rats were randomly divided into groups of alcohol with any intervention, exercise, curcumin, sham and exercise + curcumin. Endurance swimming training program was conducted for two weeks, daily and 20-60 min, equally in groups with exercise. There was daily intra-peritoneal injection of curcumin (50mg/kg weight of body), equally in groups with curcumin. Rats in sham group were injected with curcumin’s solvent (dimethyl sulfoxide). The data were analyzed by ANOVA and Tukey's post hoc test at the significant level of p< 0.05. Plasma level of ALP enzyme (p=0.0001) significantly increased in the alcohol group compared to the control group. Also the result show that the was significantly decreased in training + curcumin group in comparison with curcuminand training groups. According the finding,alcohol abuse significantly increase Plasma level of  ALP enzyme. It seems that Short-term exercise with curcumin, caninhibit enzymthat enhanced by alcohol abuse.

Keywords

Main Subjects

References

1.         Dwyer-Lindgren L, Flaxman AD, Ng M, Hansen GM, Murray CJ, Mokdad AH. Drinking patterns in US counties from 2002 to 2012. American journal of public health. 2015;105(6):1120-7.
2.         Eskandarieh S, Hajebi A, Noroozi A, Haghdoost AA, Baneshi MR. Epidemiology of alcohol abuse in Iran. Asia Pacific Journal of Medical Toxicology. 2014;3(Supplement 1):22-.
3.         Jaurigue MM, Cappell MS. Therapy for alcoholic liver disease. World journal of gastroenterology: WJG. 2014;20(9):2143.
4.         Kim M-S, Ong M, Qu X. Optimal management for alcoholic liver disease: Conventional medications, natural therapy or combination? World journal of gastroenterology. 2016;22(1):8.
5.         Salas Martins I, Teixeira Coelho L, Casajus MI, Okani ET. Smoking, consumption of alcohol and sedentary life style in population grouping and their relationships with lipemic disorders. Revista de saude publica. 1995;29:38-45.
6.         Mokdad AH, Marks JS, Stroup DF, Gerberding JL. Actual causes of death in the United States, 2000. Jama. 2004;291(10):1238-45.
7.         Maynard ME, Leasure JL. Exercise enhances hippocampal recovery following binge ethanol exposure. PloS one. 2013;8(9).
8.         Mokdad AA, Lopez AD, Shahraz S, Lozano R, Mokdad AH, Stanaway J, et al. Liver cirrhosis mortality in 187 countries between 1980 and 2010: a systematic analysis. BMC medicine. 2014;12(1):145.
9.         Ponomarenko Y, Leo MA, Kroll W, Lieber CS. Effects of alcohol consumption on eight circulating markers of liver fibrosis. Alcohol and alcoholism. 2002;37(3):252-5.
10.       Varatharajalu R, Garige M, Leckey LC, Reyes-Gordillo K, Shah R, Lakshman MR. Protective role of dietary curcumin in the prevention of the oxidative stress induced by chronic alcohol with respect to hepatic injury and antiatherogenic markers. Oxidative medicine and cellular longevity. 2016;2016.
11.       Song B-J, Akbar M, Abdelmegeed MA, Byun K, Lee B, Yoon SK, et al. Mitochondrial dysfunction and tissue injury by alcohol, high fat, nonalcoholic substances and pathological conditions through post-translational protein modifications. Redox biology. 2014;3:109-23.
12.       Xue M, Liu Y, Lyu R, Ge N, Liu M, Ma Y, et al. Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats. PloS one. 2017;12(6).
13.       Kaneko JJ, Harvey JW, Bruss ML. Clinical biochemistry of domestic animals: Academic press; 2008.
14.       Fischbach K-F, Zawta B. Age-dependent reference limits of several enzymes in plasma at different measuring temperatures. 1992.
15.       Klein EA, Thompson I, Tangen CM, Lucia MS, Goodman P, Minasian LM, et al. Vitamin E and the risk of prostate cancer: Updated results of the Selenium and Vitamin E Cancer Prevention Trial (SELECT). 2012.
16.       Malhotra N, Beaton MD. Management of non-alcoholic fatty liver disease in 2015. World journal of hepatology. 2015;7(30):2962.
17.       Al-Jiffri O, Al-Sharif F, El-Kader S, Ashmawy E. Weight reduction improves markers of hepatic function and insulin resistance in type-2 diabetic patients with non-alcoholic fatty liver. African health sciences. 2013;13(3):667-72.
18.       Nobili V, Manco M, Devito R, Di Ciommo V, Comparcola D, Sartorelli MR, et al. Lifestyle intervention and antioxidant therapy in children with nonalcoholic fatty liver disease: a randomized, controlled trial. Hepatology. 2008;48(1):119-28.
19.       Georgakouli K, Manthou E, Fatouros IG, Deli CK, Spandidos DA, Tsatsakis AM, et al. Effects of acute exercise on liver function and blood redox status in heavy drinkers. Experimental and therapeutic medicine. 2015;10(6).
20.       Chen N, Geng Q, Zheng J, He S, Huo X, Sun X. Suppression of the TGF-β/Smad signaling pathway and inhibition of hepatic stellate cell proliferation play a role in the hepatoprotective effects of curcumin against alcohol-induced hepatic fibrosis. International journal of molecular medicine. 2014;34(4):1110-6.
21.       Majchrowicz E. Induction of physical dependence upon ethanol and the associated behavioral changes in rats. Psychopharmacologia. 1975;43(3):245-54.
22.       Granados‐Castro LF, Rodríguez‐Rangel DS, Fernández‐Rojas B, León‐Contreras JC, Hernández‐Pando R, Medina‐Campos ON, et al. Curcumin prevents paracetamol‐induced liver mitochondrial alterations. Journal of Pharmacy and Pharmacology. 2016;68(2):245-56.
23.       Vesali-Akbarpour L, Samavati-Sharif MA. The effect of endurance swimming plus vitamin C supplement on oxidative stress and muscles damage indices in male wistar rats. Avicenna Journal of Medical Biochemistry. 2016;4(1):11-34241.
24.       Jefferys D, Funder J. The effect of water temperature on immobility in the forced swimming test in rats. European journal of pharmacology. 1994;253(1-2):91-4.
25.       Hyder MA, Hasan M, Mohieldein AH. Comparative levels of ALT, AST, ALP and GGT in liver associated diseases. European journal of experimental biology. 2013;3(2):280-4.
26.       Singh M, Gupta S, Singhal U, Pandey R, Aggarwal S. Evaluation of the oxidative stress in chronic alcoholics. Journal of clinical and diagnostic research: JCDR. 2013;7(8):1568.
27.       Teschke R. Alcoholic liver disease: alcohol metabolism, cascade of molecular mechanisms, cellular targets, and clinical aspects. Biomedicines. 2018;6(4):106.
28.       Shamsoddini A, Sobhani V, Chehreh MEG, Alavian SM, Zaree A. Effect of aerobic and resistance exercise training on liver enzymes and hepatic fat in Iranian men with nonalcoholic fatty liver disease. Hepatitis monthly. 2015;15(10).
29.       Alie M, Matinhommaee H, Azarbayjani M, Peeri M. The effect of resistance training intensity on enzymatic and nonenzymatic markers of liver function in obese males. Ind J Fund Appl Life sci. 2015;5(2):101-10.
30.       Barzegarzadeh-Zarandi H, Dabidy-Roshan V. Changes in some liver enzymes and blood lipid level following interval and continuous regular aerobic training in old rats. Journal of Shahrekord Uuniversity of Medical Sciences. 2012;14.
31.       Tartibian B, Motabsaee N, Tolouei-Azar J. The influence of nine-week intensive aerobic exercises, calcium and vitamin D supplementation on the metabolic response of bone formation biomarkers. Zahedan Journal of Research in Medical Sciences. 2013;15(2).
32.       Skrypnik D, Ratajczak M, Karolkiewicz J, Mądry E, Pupek-Musialik D, Hansdorfer-Korzon R, et al. Effects of endurance and endurance–strength exercise on biochemical parameters of liver function in women with abdominal obesity. Biomedicine & Pharmacotherapy. 2016;80:1-7.
33.       Ekun OA, Emiabata AF, Abiodun OC, Ogidi NO, Adefolaju FO, Ekun OO. Effects of football sporting activity on renal and liver functions among young undergraduate students of a Nigerian tertiary institution. BMJ open sport & exercise medicine. 2017;3(1):e000223.
34.       Rukkumani R, Aruna K, Varma PS, Rajasekaran KN, Menon VP. Comparative effects of curcumin and an analog of curcumin on alcohol and PUFA induced oxidative stress. J Pharm Pharm Sci. 2004;7(2):274-83.
35.       Salehpoor G, Rezaei S, Salehi I. The effect of fatigue and clinical course on depression, anxiety, stress, quality of life and employment status in patients with multiple sclerosis. Daneshvar. 2012;20(5):51-62.
36.       Feng W-w, Kuang S-y, Tu C, Ma Z-j, Pang J-y, Wang Y-h, et al. Natural products berberine and curcumin exhibited better ameliorative effects on rats with non-alcohol fatty liver disease than lovastatin. Biomedicine & Pharmacotherapy. 2018;99:325-33.

Files

History

  • Receive Date: 26 February 2020
  • Revise Date: 01 May 2020
  • Accept Date: 07 May 2020

Share

How to cite

Statistics